Table 2.
Stem loop, primers, and probe used for specific synthesis of cDNA and performing quantitative PCR regarding miRNA amplification
| Primer types | miRNAs | Sequence 5 → 3 |
|---|---|---|
| Stem loop | miR-155-5p | GTATGCTGCTACCTCGGACCCTGCTTAGTGCCATGCCTGCCATCGAGCAGCATAC AACCCC |
| miR-146a | GTATGCTGCTACCTCGGACCCTGCTTAGTGCCATGCCTGCCATCGAGCAGCATAC AACCCA | |
| U6 | GTATGCTGCTACCTCGGACCCTGCTTAGTGCCATGCCTGCCATCGAGCAGCATACAAAAATATGG | |
| Forward | miR-155-5p | GCGCCTGAGAACTGAATTCCATGGGTT |
| miR-146a | GCGCCTGAGAACTGAATTCCATGGGTT | |
| U6 | GCAAGGATGACACGCAAATTCG | |
| Probe | TaqMan probe | AGTGCCATGCCTGCCATCGAGC |
| Reverse | Universal | GCTGCTACCTCGGACCCT |