Table 3.

The effect of leptin and glucantime, or their combination (Lep + Glu), on the Leishmania tropica amastigote

Types of cultures	No. of intracellular amastigotes (mean ± SD)	P values*	P value**
Untreated control	31.6 ± 2.1	–	–
Lep-2.5	26.6 ± 1.8	< 0.001	< 0.001
Lep-2.5 + Glu-100	14.3 ± 0.7	< 0.001
Lep-5	19.7 ± 0.4	< 0.001	< 0.001
Lep-5 + Glu-100	7.3 ± 0.3	< 0.001
Lep-10	13.1 ± 0.2	< 0.001	< 0.001
Lep-10 + Glu-100	0.0 ± 0.0	< 0.001
Lep-20	4.2 ± 0.1	< 0.001	< 0.001
Lep-20 + Glu-100	0.0 ± 0.0
Glu 50	23.3 ± 1.1	< 0.001	–
Glu-100	16.8 ± 0.8	< 0.001	–
Glu-200	8.4 ± 0.6	< 0.001	–
Glu 400	0.0 ± 0.0	< 0.001	–

^*P values in compared with untreated control culture

^**P values regarding differences between Lep-treated culture and Lep + Glu-treated culture