Table 1.

Primer sequences used for qRT-PCR and methylation sequencing

Gene	Sequence (5’ to 3’)	Application
STAT3	Forward: ACCCAACAGCCGCCGTAG Reverse: CAGACTGGTTGTTTCCATTCAGAT	qRT-PCR
RORc	Forward: TGGGCTCCAAGAGAAGAGGA Reverse: CAGGCTCCGGAGTTTTCCTT	qRT-PCR
RORγt	Forward: GACCCACACCTCACAAATTGA Reverse: AGTAGGCCACATTACACTGCT	qRT-PCR
IL-17 A	Forward: GAGCTTCATCTGTGTCTCTGA Reverse: GCCAAGGGAGTTAAAGACTTTG	qRT-PCR
GAPDH	Forward: GGTGAAGGTCGGTGTGAACG Reverse: CTCGCTCCTGGAAGATGGTG	qRT-PCR
STAT3	Forward: GGAGGTTTTAGGGTGGTTTAGTTAG Reverse: CCCCATATAAATATCCATTCACAAC	Methylation Sequencing
RORc	Forward: TGATTGAGAATTTGGTTTTTTGTTT Reverse: AAAACATCCCTACCCCTAATATCAC	Methylation Sequencing