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. Author manuscript; available in PMC: 2025 Aug 27.
Published in final edited form as: J Am Soc Mass Spectrom. 2024 May 16;35(6):1253–1260. doi: 10.1021/jasms.4c00067

Figure 2. The impact of column parameters and n-Dodecyl-β-D-maltoside (DDM) on timsTOF SCP sensitivity.

Figure 2.

The default parameters were configured as follows: 30min gradient time, 166ms ramp time and a 20 m/z isolation window. (A) Percentage of identified precursors and (B) percentage of identified proteins with different loading amount of HeLa cell digest (n = 3). 100% equivalents to 33,434 precursors in Fig 2A and 3,868 proteins in Fig 2B.