Table 2.
Survival of ’lactobacilli’ at different pH and bile concentrations
| Strains No. | Organism best match | Acid tolerance (%) | Bile tolerance (%) | |||
|---|---|---|---|---|---|---|
| pH 2 | pH 3 | 0.3 | 0.5 | 1 | ||
| ST1 | Levilactobacillus brevis | 47.33 ± 0.1dB | 69.34 ± 0.07 bA | 81.96 ± 0.06 cA | 47.22 ± 0.11fB | 47.34 ± 0.05 dB |
| ST2 | Levilactobacillus brevis | 45.92 ± 0.13 eB | 63.86 ± 0.11 cA | 92.48 ± 0.03 aA | 47.28 ± 0.04 fB | 47.22 ± 0.12 dB |
| ST3 | Lecticaseibacillus paracasei | 9.98 ± 0.05 lB | 14.95 ± 0.06 mA | 92.45 ± 0.08 aA | 47.37 ± 0.09 fB | 47.34 ± 0.06 dB |
| ST4 | Lentilactobacillus hilgardii | 23.77 ± 0.08 jB | 57.08 ± 0.17 eA | 77.88 ± 0.04 dA | 23.84 ± 0.05 oB | 23.48 ± 0.03 lB |
| ST5 | Lentilactobacillus hilgardii | 47.12 ± 0.17 dB | 61.54 ± 0.04 dA | 83.98 ± 0.03 bA | 40.76 ± 0.08 gB | 40.65 ± 0.07fB |
| ST6 | Lentilactobacillus hilgardii | 54.79 ± 0.05 bB | 69.36 ± 0.17bA | 81.94 ± 0.08cA | 64.83 ± 0.04cB | 54.78 ± 0.04cC |
| ST7 | Lentilactobacillus parabuchneri | 47.63 ± 0.08dB | 61.47 ± 0.89dA | 73.65 ± 0.06fA | 65.43 ± 0.04bB | 65.37 ± 0.05aB |
| ST8 | Lecticaseibacillus paracasei | 65.47 ± 0.11aB | 70.46 ± 0.06aA | 73.68 ± 0.11fA | 64.45 ± 0.06dB | 55.34 ± 0.06cC |
| ST9 | Lentilactobacillus hilgardii | 45.77 ± 0.04eB | 53.55 ± 0.06fA | 66.43 ± 0.04gA | 40.64 ± 0.05gB | 38.33 ± 0.04gC |
| ST10 | Lecticaseibacillus paracasei | 49.78 ± 0.05cB | 52.69 ± 0.08gA | 76.22 ± 0.02eA | 72.02 ± 0.03aB | 60.85 ± 0.07bC |
| ST11 | Lecticaseibacillus paracasei | 43.26 ± 0.15fB | 69.55 ± 0.08bA | 64.75 ± 0.07hA | 25.54 ± 0.09mB | 24.69 ± 0.30kC |
| ST12 | Lecticaseibacillus paracasei | 42.19 ± 0.11gB | 47.64 ± 0.06hA | 32.34 ± 0.05mC | 39.13 ± 0.04iB | 41.43 ± 0.04eA |
| ST13 | Lecticaseibacillus paracasei | 17.08 ± 0.02kB | 23.00 ± 0.04lA | 42.12 ± 0.03jA | 39.95 ± 0.07hB | 38.51 ± 0.70gC |
| ST14 | Lecticaseibacillus paracasei | 5.04 ± 0.07mB | 11.02 ± 0.08nA | 21.74 ± 0.08oC | 28.22 ± 0.02lB | 33.48 ± 0.17iA |
| ST15 | Lecticaseibacillus paracasei | 34.15 ± 0.11iB | 41.18 ± 0.04jA | 34.13 ± 0.04lB | 32.26 ± 0.08jC | 35.58 ± 0.40hA |
| ST16 | Lecticaseibacillus paracasei | 31.49 ± 0.08jB | 32.93 ± 0.04kA | 30.77 ± 0.05nA | 30.13 ± 0.04kB | 30.22 ± 0.16jB |
| ST17 | Levilactobacillus brevis | 41.91 ± 0.13gB | 48.02 ± 0.11hA | 37.35 ± 0.07kA | 25.23 ± 0.04nC | 30.92 ± 0.17jB |
| ST18 | Lecticaseibacillus paracasei | 36.44 ± 0.06hB | 43.79 ± 0.10iA | 58.95 ± 0.07iA | 49.66 ± 0.08eB | 40.36 ± 0.06fC |
The pH values and bile concentrations represent the means of three replicates. Student t-test was used for comparison of the mean survival at pH 2 and 3. Values with different superscript letters (a-o) in the same column and different superscripts with capital letter across the row are significantly different (p < 0.05). Multiple comparisons for bile tolerance were done using Tukey’s HSD