Table 1.
Kronos mrc mutants used in this study
| Genotype | Mutation | SIFT score | PROVEAN score | Comments: |
|---|---|---|---|---|
| Missense mutants included in the study (29) | ||||
| K3745 | A19T | 0.18 | − 0.856 | Exp. 1 |
| K3543 | G63S | 0.7 | 0.926 | Exp. 1 |
| K1435 | S92N | 0.06 | 0.004 | Exp. 1 |
| K2447 | L149F | 0* | − 3.944* | Exp. 1 |
| K2937 | A178T | 0.33 | − 2.114 | Exp. 1; Exp. 2 |
| K2124 | R196K | 0.75 | 0.12 | Exp. 1 |
| K2205 | E213K | 0.15 | − 2.437 | Exp. 1 |
| K3499 | R230C | 0.16 | − 2.755* | Exp. 1 |
| K1431 | E225K | 0* | − 3.911* | Exp. 1 |
| K2397 | R230C | 0.04* | − 2.755* | Exp. 1 |
| K4060 | R255K | 0.38 | 0.05 | Exp. 1; Exp. 2 |
| K0598 | L289F | 0* | − 3.961* | Exp. 1; Exp. 2 |
| K3533 | A346V | 0.13 | − 3.278* | Exp. 1 |
| K2981 | A356T | 0.11 | − 0.575 | Exp. 1 |
| K0775 | L394F | 0* | − 3.5* | Exp. 1 |
| K0265 | M412I | 0.03* | − 2.153 | Exp. 1; Exp. 2 |
| K3424 | E491K | 0.01* | − 3.267* | Exp. 1 |
| K2408 | S503N | 1 | 3.094 | Exp. 1; Exp. 2 |
| K1232 | S539N | 1 | 1.528 | Exp. 1 |
| K3889 | D581N | 0.13 | − 2.011 | Exp. 1; Exp. 2 |
| K3505 | E606K | 0.22 | − 1.96 | Exp. 1; Exp. 2 |
| K2485 | A625T | 0.01* | − 2.79* | Exp. 1; Exp. 2 |
| K4297 | P656L | 0.13 | − 1.238 | Exp. 1 |
| K3416 | S667N | 0.24 | 0.188 | Exp. 1 |
| K2348 | E679K | 0.02* | − 0.233 | Exp. 1 |
| K2096 | P681S | 0.01* | 0.835 | Exp. 1 |
| K2821 | A687V | 0.13 | − 3.182* | Exp. 1 |
| K3666 | D708N | 0.02* | − 1.631 | Exp. 1 |
| K4193 | S726N | 0.46 | − 0.47 | Exp. 1 |
| Missense mutants excluded in the study (16) | ||||
| K4533 | A79V | 0.01* | − 3.263* | Insufficient seeds |
| K2450 | A155T | 0.12 | − 0.42 | Poor growth |
| K4618 | R196K | 0.75 | 0.12 | Insufficient seeds |
| K0565 | S329F | 0.01* | − 3.214* | Unable to genotype |
| K0665 | A356T | 0.11 | − 0.575 | Insufficient seeds |
| K3286 | S375F | 0.01* | − 5.338* | Poor growth |
| K2976 | L409F | 0.07 | − 0.445 | Insufficient seeds |
| K1112 | E413K | 0.15 | − 3.465* | Poor growth |
| K3145 | E425K | 0.04* | − 3.8* | Poor growth |
| K3657 | T500I | 0.07 | − 1.68 | Unable to genotype |
| K1161 | T525I | 0.1 | − 3.878* | Poor growth |
| K3661 | D575N | 0.86 | 0.287 | Poor growth |
| K4205 | L591F | 0.24 | − 0.127 | Poor growth |
| K3137 | A603T | 1 | 2.048 | Unable to genotype |
| K0338 | R700K | 0.48 | − 0.325 | Insufficient seeds |
| K4082 | D743N | 0.52 | − 1.675 | Unable to genotype |
| Premature stop codon mutants included in the study (2) | ||||
| mrc-1 | W258STOP | – | Exp. 1; Exp. 2 | |
| mrc-2 | W550STOP | – | Exp. 1 | |
SIFT and PROVEAN scores that are considered damaging (using default cut-off values of 0.05 and − 2.5 for each programme, respectively) are marked with an asterisk (*). For lines included in the study, the experiment(s) in which phenotypic analyses were conducted is provided in the comments. For lines excluded, the reason for exclusion is provided in the comments