Fig. 2.

FF-DMTS viability experiments on primary rat brain endothelial cells. The effect of FF-DMTS on cellular viability after 10 min treatment in Ringer-HEPES buffer was measured by a MTT test or b LDH assay. c and d Real-time cell electronic sensing measurement. Cell index is calculated from the impedance of cell layers which reflects adherence, barrier integrity, and viability of the cultured brain endothelial cells. Due to the treatment duration on the 96-well plate, the first measured time point was 20 min. c Kinetics of impedance until 60 min; d Normalized cell index values at 20-min time-point. Abbreviations: TX = Triton X-100 detergent as 100% cell death control. MTT: 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide; LDH: lactate dehydrogenase. One-way ANOVA with Dunnett’s post-test;*, p < 0.05; **, p < 0.01; ***, p < 0.001, compared to the control, n = 4–8