Fig. 3.
Barrier integrity and permeability measurements on the blood–brain barrier co-culture model after 10 min FF-DMTS treatments. a Transendothelial electrical resistance (TEER) was measured before and after the experiment. Two-way ANOVA with Dunnett’s post-test, ###, p < 0.001, compared to before treatment value; ***, p < 0.001, compared to the 30 µg/ml treatment concentration, n = 4. b Sodium fluorescein (SF) permeability was assessed after FF-DMTS treatment of the BBB model. Pe: endothelial permeability coefficient. Unpaired t-test, ***, p < 0.001, compared to the control, 30 µg/ml treatment concentration, n = 4. c Permeability of DMTS through BBB co-culture model after treatments with 30 and 100 µg/ml FF-DMTS. N.D.: not detected. d Immunofluorescent images of zonula occludens-1 (ZO-1) junctional associated protein after FF-DMTS treatments (0, 30, 100 µg/ml) on primary brain endothelial cells. Bar: 100 µm