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. 2025 Jul 3;45(9):1616–1635. doi: 10.1161/ATVBAHA.124.322893

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© 2025 The Authors.

Arteriosclerosis, Thrombosis, and Vascular Biology is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 3. — Generation of transgenic Lmna-OE mice with Cre-dependent overexpression of lamin A in hematopoietic cells. A, Representation of the genetic construct inserted in the mouse Rosa26 locus before and after Cre-dependent removal of the LoxP-flanked stop sequence. B, Relative Lmna transcript expression in circulating leukocytes, normalized to the average expression of 2 housekeeping genes (Rplp0 and Actb; N=4 Lmna^tg; n=8 Lmna-OE; each dot represents a pool of 2 or 3 animals, with approximately equal numbers of males and females). Statistical significance was assessed by the 2-tailed unpaired t test. C, Lamin A protein expression in circulating leukocytes assessed by flow cytometry (n=3 male and 2 female mice per genotype). Statistical significance was determined by the multiple t test with the Holm-Sidak method, with α=0.05. Each cell population was analyzed individually, without assuming a consistent SD. D, Lamin A protein expression in the Lin⁻ Sca1⁻ cKit⁺ (LK) and Lin⁻ Sca1⁺ cKit⁺ (LSK) bone marrow progenitor populations, assessed by flow cytometry and presented as mean fluorescence intensity (MFI); n=3 male and 2 female mice per genotype. Statistical significance was determined by the 2-tailed unpaired t test. E, Immunoblot analysis of lamin A/C in circulating leukocytes from mice of the indicated genotype. Endogenous lamin A runs at the expected 72 KDa in all mice, while Lmna-OE mice overexpress a larger lamin A isoform (lamin A^+51aa). The graphs show the quantification of lamin A and lamin A+A^+51aa, normalized to GAPDH as loading control. Statistical significance was determined using a 2-tailed unpaired t test comparing Lmna^tg and Lmna-OE. F, Representative immunofluorescence images of peripheral blood leukocytes from Lmna^tg and Lmna-OE mice showing the perinuclear staining characteristic of lamin A and increased lamin A protein expression in Lmna-OE mice. MERGE refers to the composite image generated by overlapping the DAPI and the Lamin A/C signals, allowing their visualization within the same field of view. cKit indicates cellular Kit proto-oncogene; Cre, causes recombination; DAPI, 4′,6-diamidino-2-phenylindole; HSC, hematopoietic stem cell; Lin, lineage; LoxP, Locus of X (cross)-over in P1; Sca1, stem cell antigen; and WT, wild type.