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. 2025 Jul 3;45(9):1616–1635. doi: 10.1161/ATVBAHA.124.322893

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© 2025 The Authors.

Arteriosclerosis, Thrombosis, and Vascular Biology is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 4. — Lamin A overexpression in hematopoietic cells reduces atherosclerosis development in atheroprone Ldlr^−/− mice. A, Protocol for atherosclerosis studies. B, Lamin A protein expression in CD45.2⁺ cells assessed by flow cytometry (Lmna^tg mice: n=8 females and n=7 males; Lmna-OE mice: n=7 females and n=8 males). Statistical significance was determined by the multiple t test with the Holm-Sidak method, with α=0.05. Each population was analyzed individually, without assuming a consistent SD. C, Transplant efficiency shown as the percentage of CD45.2⁺ circulating leukocytes 4 weeks after bone marrow transplantation (BMT), evaluated by flow cytometry (Lmna^tg mice: n=8 females and n=7 males; Lmna-OE mice: n=7 females and n=8 males). Statistical significance was determined using a Mann-Whitney U test. D, Body weight evolution over the 6-week fat-feeding period (Lmna^tg mice: n=8 females and n=7 males; Lmna-OE mice: n=7 females and n=8 males). Statistical significance was determined by ANOVA. E, Absolute cell counts in peripheral blood of reconstituted animals after 6 weeks of fat feeding (Lmna^tg mice: n=8 females and n=7 males; Lmna-OE mice: n=7 females and n=8 males). Statistical significance was determined by the multiple t test with the Holm-Sidak method, with α=0.05. Each cell population was analyzed individually, without assuming a consistent SD. F, Fasting plasma lipid concentration after 6 weeks of fat feeding (Lmna^tg mice: n=8 females and n=7 males; Lmna-OE mice: n=7 females and n=8 males). Statistical significance was determined by the multiple t test with the Holm-Sidak method, with α=0.05. Each cell population was analyzed individually, without assuming a consistent SD. G, Representative images of Oil Red O–stained aortas and quantification of plaque burden in the aortic arch (Lmna^tg mice: n=15; Lmna-OE mice: n=14). Statistical differences were assessed by the 2-tailed unpaired t test. H, Representative images of Oil Red O–stained right carotid arteries and quantification of plaque burden (n=5 for Lmna^tg and n=5 for Lmna-OE). Statistical differences were assessed by the 2-tailed unpaired t test. BM indicates bone marrow; HDL, high-density lipoprotein; HFD, high-fat diet; LDL, low-density lipoprotein; MFI, mean fluorescence intensity; TG, triglyceride; and WBC, white blood cell.