Figure 6.

Characterization of immune cell reclustering in the atherosclerotic aorta of Ldlr^−/− mice with lamin A/C deficiency or lamin A overexpression in hematopoietic cells. A, Uniform manifold approximation and projection representation of single-cell RNA sequencing (scRNA-seq) immune cell reclustering (CD45⁺), showing cell clusters and identified cell types in Ldlr^−/− mice reconstituted with bone marrow of the indicated genotype and fed the high-fat diet for 6 weeks. B, Bar plots representing the proportions of each immune cell type in each condition (in percentages). Colors for each cluster are defined as in A. C, Violin plots showing gene signature scores for neutrophil activation, NETosis, and apoptosis in the neutrophil cluster (IC6). The black horizontal line marks the mean score for each BM genotype. Statistical significance was determined by ANOVA with the Tukey post hoc test or with Kruskal-Wallis with Bonferroni correction. D, Biological processes altered in aortic macrophage clusters IC3 (left) and IC4 (right) in Ldlr^−/− mice reconstituted with Lmna^−/− BM and fed the high-fat diet for 6 weeks versus wild-type (WT) control bone marrow. Statistical significance was determined by the Benjamini-Hochberg procedure. Discontinuous black lines indicate Benjamini-Hochberg P=0.05. BM indicates bone marrow; GO, gene ontology; and IC, immune cluster.