Fig. 2.
Effects of paternal treatments on methylation of rDNA in offspring tissues. A. Methylation at CpG sites in male offspring lung. Compared with UT-lineage, a, P = 0.012, b, P = 0.039, c, P = 0.037, d, P = 0.023, e, P = 0.042, all sites together, p = 0.028, after correction for variables (see Methods). B. Methylation level was higher in lung than in liver, and this difference was greater in Cr-lineage offspring, b, P = 0.0047, c, P = 0.0069 compared with UT-lineage, based on within-animal comparisons (see Methods). C. Methylation level was higher in sperm than in lung, and this difference was greater in Cr-lineage offspring, d, P < 0.0001, and in AS-lineage offspring, e, P = 0.0002, compared with UT-lineage. D. Methylation was higher in sperm than in liver, a difference that was not significantly affected by paternal treatments.
