Table 1. Primer sequences used to determine the expression of genes encoding tight junction proteins using quantitative reverse transcription-PCR.

Target gene		Primer sequence (5′→3′)	Reference
claudin-1	Forward	AAGTGCTTGGAAGACGATGA	[63]
	Reverse	CTTGGTGTTGGGTAAGAGGTT
occludin	Forward	CCAATGTCGAGGAGTGGG
	Reverse	CGCTGCTGTAACGAGGCT
ZO-1	Forward	ATCCCTCAAGGAGCCATTC
	Reverse	CACTTGTTTTGCCAGGTTTTA
β-actin	Forward	TTTTAGGATGGCAAGGGACTT
	Reverse	GATGAGTTGGCATGGCTTTA

PCR, polymerase chain reaction.