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. 2025 Aug 13;19:1641801. doi: 10.3389/fnhum.2025.1641801

TABLE 2.

Manipulation check outcomes for alpha-amylase (AA), heart rate (HR), cortisol (CORT), and situational anxiety (STAI-S) scores, including mean differences and the results of independent samples t-tests, with effect size in Cohen’s D.

Outcome measure Mean difference t-test result
Alpha amylase 39.1 U/mL t(44) = 3.01, p < 0.01, Cohen’s D = 1.54
Heart rate 19.84 BPM t(34) = 9.09, p < 0.001, Cohen’s D = 0.44
Respiration rate 0.61 BPM t(36) = 1.13, p = 0.27, Cohen’s D = 0.19
Cortisol 0.28 μg/dL t(45) = 3.39, p < 0.01, Cohen’s D = 0.5
STAI-S 8.52 points t(45) = 6.32, p < 0.001, Cohen’s D = 0.93