REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-Bin1 1:100 (IF), 1:1000 (WB), 1:80 (IHC)	Millipore	Cat#05-449; RRID:AB_309738
Rabbit monoclonal anti-Bin1 1:100 (IF)	Abcam	Cat#ab182562; RRID:AB_3669062
Rabbit polyclonal anti-synapsin I 1:250 (IF), 1:1000 (WB)	Abcam	Cat#ab8; RRID:AB_2200097
Rabbit polyclonal anti-PSD-95 1:200 (IF), 1:1000 (WB)	Millipore	Cat#AB9708; RRID:AB_2092543
Guinea Pig polyclonal anti-vGLUT1 1:200 (IF), 1:500 (IHC)	SynapticSystems	Cat#135 304; RRID:AB_887878
Rabbit polyclonal anti-vGAT 1:500 (IF), 1:200 (IHC)]	Merck Millipore	Cat#AB5062P; RRID:AB_2301998
Mouse monoclonal anti-gephyrin 1:250 (IF)	SynapticSystems	Cat#147 011; RRID:AB_2810215
Mouse monoclonal anti-tubulin 1:10,000 (WB)	Millipore	Cat#MAB1637; RRID:AB_2210524
Goat monoclonal anti-GAPDH 1:10000 (WB)	Sicgen	Cat#ab0049; RRID:AB_2333142
Mouse monoclonal anti-c-Myc 1:500 (IF)	Thermo Fisher Scientific	Cat#13-2500; RRID:AB_2533008
Goat polyclonal anti-Rab5 1:200 (IF)	Sicgen	Cat#ab1024; RRID: AB_2333166
Rabbit polyclonal anti-SV2A 1:200 (IF)	SynapticSystems	Cat#119 002; RRID:AB_887802
Chemicals, peptides, and recombinant proteins
Heat-Inactivated FBS	Thermo Fisher Scientific	Cat#16140-071
poly-D-lysine	Sigma-Aldrich	Cat#P1149
Brainphys Neuronal Medium-SM1	STEMCELL Technologies	Cat#05792
Neurobasal medium	Thermo Fisher Scientific	Cat#21103049
B-27	Thermo Fisher Scientific	Cat#17504044
Glutamax	Thermo Fisher Scientific	Cat#35050-038
penicillin-streptomycin	Thermo Fisher Scientific	Cat#15140-122
Lipofectamine2000	Thermo Fisher Scientific	Cat#11668019
Fetal Bovine Serum	Sigma- Aldrich	Cat#F0804
DAPT	Calbiochem	Cat#565770
β-Secretase Inhibitor IV	Calbiochem	Cat#565788
DMSO	PanReac AppliChem	Cat#A3672.0100
Levetiracetam	Sigma-Aldrich	Cat#L8668
Lenti-X concentrator	Takara	Cat#631231
Fura-Red	Thermo Fisher Scientific	Cat#F3021
6-cyano-7-nitroquinoxaline-2,3-dione	Sigma-Aldrich	Cat#C127
D, L-2-amino-5-phosphonovaleric acid	Sigma-Aldrich	Cat#A5282
FM4-64FX	Thermo Fisher Scientific	Cat#F34653
Fluoromount-G	Southern Biotechnology	Cat#00-4958-02
DAPI	Sigma	Cat#D9542
Experimental models: Cell lines
N2a	Z. Lenkei (IPNP)	ATCC®CCL-131™
Experimental models: Organisms/strains
Mouse/ BALB/c	Charles River	BALB/cByJ
Recombinant DNA
vGAT-pHluorin	SH Kim (Kyung Hee University)	N/A
vGLUT1-pHluorin	SH Kim	N/A
pCI syn iGluu	Addgene	Cat#106122
pGP-CAG-iGABASnFR2-WPRE-bGH-polyA	Addgene	Cat#218868
nBin1-myc	In house	27
nBin1PL-myc	In house
nBin1KR-myc	In house
shBin10	Sigma	TRCN0000380696
shBin11	Sigma	TRCN0000380439
shBin12	Sigma	TRCN0000088191
TurboGFP™ Positive Control Plasmid DNA	Sigma	Cat#SHC003
pGFP-C-shLenti	OriGene Technologies	Cat#NC1766994
psPAX2	D Trono(EPFL)	Cat#12260
pMD2.G	D Trono(EPFL)	Cat#12259
Software and algorithms
Fiji (version 1.53)	NIH	https://fiji.sc/85
Time Series Analyzer-V3	Balaji-J	https://imagej.net/ij/plugins/time-series.html
ICY	Institut Pasteur and France-BioImaging	Icy.bioimageanalysis.org86
IMARIS (version 9.5.0)	Oxford Instruments	https://imaris.oxinst.com/
ComDet v.0.5.5	Katrukha	https://github.com/ekatrukha/ComDet
GraphPad (version 8.4.0)	Dotmatics	www.graphpad.com
CellProfiler (version 4.2.1)	Cimini lab (Broad Institute)	www.cellprofiler.org87