Immobilized Listeria monocytogenes cells. Cells were harvested by centrifugation at 10,000 × g for 5 min, washed, and suspended in 30 μl of MM. An aliquot of the culture was applied to a coverslip cleaned and coated with poly-l-lysine and was allowed to settle for 2 to 3 min, after which the unattached bacteria were washed off under running MM. Bar, 5 μm.