FIG. 2.

Culturable indigenous bacteria determined on days 0, 1, 4, 6, 8, 14, and 33 after treating the Bordeaux A1 and A2 soils with the organic solvents acetone and dichloromethane according to two different protocols: partial treatment protocol with acetone (□) or dichloromethane (▵) and full treatment protocol with acetone (○) or dichloromethane (▿). Controls (⧫) did not receive any solvents. Three different media were used to detect different groups of bacteria: the oligotrophic medium 1/300 TSA (A and B), the rich medium LB (C and D), and the Pseudomonas spp.-specific medium Gould's S1 (E and F). The detection limit is indicated by a stippled line. (A, C, and E) Bordeaux A1 (0- to 20-cm depth). (B, D, and F) Bordeaux A2 (40- to 60-cm depth). Each data point represents the mean (± SD) of three independent replicates. Significant differences from the control are indicated by asterisks (P < 0.05). To simplify panel F, SD is only indicated when positive.