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. 2025 Aug 21;13(8):2036. doi: 10.3390/biomedicines13082036
HKG(s) Housekeeping gene(s)
RT-qPCR Reverse transcription quantitative polymerase chain reaction
RNA Ribonucleic acid
cDNA Complementary deoxyribonucleic acid
36B4 Ribosomal protein, large, P0
HPRT Hypoxanthine-guanine phosphoribosyl transferase
Actb Actin, beta
HMBS Hydroxymethylbilane synthase
GAPDH Glyceraldehyde-3-phosphate dehydrogenase
18S 18S ribosomal RNA
PPARγ Peroxisome proliferator-activated receptor gamma, transcript variant 2
L. paracasei Lacticaseibacillus paracasei
MIQE Minimum Information for Publication of Quantitative Real-Time PCR Experiments
DMEM Dulbecco’s modified Eagle’s medium
FBS Fetal bovine serum
PBS Phosphate-buffered saline
BM Basal medium
AIM Adipogenic induction media
AMM Maintenance media
MRS de Man, Rogosa, and Sharpe broth-treated induced control group
IC Untreated, induced control group
M2.1, C8, C15, P4 Experimental group of induced adipocytes treated with 10% v/v supernatants from the respective L. paracasei strain
SD Standard deviation
CV Coefficient of variation
r Pearson correlation coefficient
Ct Cycle threshold
∆Ct delta Ct
∆∆Ct delta delta Ct