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. 2002 Apr;68(4):2061–2065. doi: 10.1128/AEM.68.4.2061-2065.2002

FIG. 1.

FIG. 1.

ITS PCR products generated from the DNA of various free-living amoebae using conserved primers only (c), conserved and species-specific primers (m), and species-specific primers only (s). (A) PCR results for three thermophilic Naegleria species. The N. fowleri long-ITS variant (fow L) and short-ITS variant (fow S) gave single bands of 453 and 409 bp, respectively, with the conserved primers and single bands of 311 bp with only the species-specific primers. With the multiplex PCR, the N. fowleri long-ITS variant displayed three bands of 453, 388 + 376, and 311 bp, whereas the N. fowleri short-ITS variant displayed four bands of 409, 376, 344, and 311 bp. N. australiensis (aust) and N. lovaniensis (lova) gave single fragments of approximately 400 bp with the conserved and the multiplex primers. (B) No PCR fragment was observed with Nuclearia spp. (Nuc) or Acanthamoeba spp. (Aca). Conserved and multiplex PCR gave single fragments of approximately 800, 600, and 500 bp with Hartmannella spp. (Har), Vahlkampfia spp. (Vah), and with Willaertia spp. (Wil), respectively. M, molecular markers (100-bp DNA ladder with a prominent 500-bp fragment).