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MseI and NlaIV digestion patterns of the ITS PCR products for the long-ITS variant of N. fowleri (lane 1), the short-ITS variant of N. fowleri (lane 2), N. lovaniensis (lane 3), and N. australiensis (lane 4). M, 100-bp DNA ladder. On the right are the restriction maps of MseI and NlaIV for the long and the short N. fowleri variants (fow L and fow S), N. lovaniensis (lova), and N. australiensis (aust). The conserved sites are marked with asterisks.
