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Addition of proline restores wild-type phenotype of C. trifolii expressing activated Ras (Val2) when grown on water agar. (A) Wild-type C. trifolii on water agar. (B) Val2 mutant on water agar. (C) Val2 mutant on water agar containing 1.6 mM proline. Note the restoration of hyphal morphology and sporulation. All colonies were photographed 7 days postinoculation at ×400 magnification.
