TABLE 3.
Enzyme activities during exclusively anaerobic growth on glucose-rich mediaa
| Strain | Headspace | IPTG (mM) | Sp act (U/mg of protein) ofb:
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| ACK | FR | GK | ICDH | ICL | PPC | PYC | |||
| NZN111 | CO2 | 0.0 | 0.20 ab | 0.17 a | 0.018 a | 0.00 | 0.00 | 0.10 a | 0.00 a |
| NZN111/pTrc99A-pyc | CO2 | 0.0 | 0.15 a | 0.26 b | 0.025 a | 0.00 | 0.00 | 0.061 b | 0.050 b |
| NZN111/pTrc99A-pyc | CO2 | 1.0 | 0.27 b | 0.33 c | 0.11 b | 0.00 | 0.00 | 0.020 c | 0.086 bc |
| AFP111 | CO2 | 0.0 | 1.11 c | 0.45 cd | 0.98 c | 0.00 | 0.00 | 0.13 d | 0.00 ad |
| AFP111/pTrc99A-pyc | CO2 | 0.0 | 1.24 c | 0.52 d | 1.08 d | 0.00 | 0.00 | 0.11 ad | 0.056 bd |
| AFP111/pTrc99A-pyc | CO2 | 1.0 | 1.42 d | 0.68 e | 1.30 e | 0.00 | 0.00 | 0.057 b | 0.12 c |
| AFP111/pTrc99A-pyc | H2 | 1.0 | 1.79 e | 0.74 e | 1.58 f | 0.00 | 0.00 | 0.099 a | 0.17 e |
a
Serum bottles were under an atmosphere of CO2 or H2.
b
Enzyme activities followed by different letters were statistically significantly different at the 90% confidence level. Enzyme abbreviations: ACK, acetate kinase; FR, fumarate reductase; GK, glucokinase; ICDH, isocitrate dehydrogenase; ICL, isocitrate lyase; PPC, PEP carboxylase; PYC, pyruvate carboxylase.