TABLE 1.
Primera | Position | Orientation | PCR specificity | Sequence |
---|---|---|---|---|
F24 | 9-27 | Forward | Universal | GAGTTTGATYMTGGCTCAG |
F25 | 1525-1541 | Reverse | Universal | AAGGAGGTGWTCCARCC |
C97 | 276-291 | Forward | Helicobacter | GCTATGACGGGTATCC |
C05 | 1477-1495 | Reverse | Helicobacter | ACTTCACCCCAGTCGCTG |
Primers F24 and F25 were used for PCR of genomic DNA for cycle sequencing. Primers C97 and C05 were used to amplify a 1.2-kb product from fecal samples for cloning.