FIG. 6.

HPLC analysis of products generated from debranched arabinan by ABN-TS and PPase-C. Debranched arabinan (0.1%) was incubated with ABN-TS (1.26 U) and PPase-C (1.18 U) in 1 ml of 20 mM sodium acetate buffer (pH 6.0) at 70 and 50°C, respectively. At 0 min (a), 1 min (b), 2 min (c), 5 min (d), 30 min (e), and 2 h (f), reaction mixtures were removed and boiled for 5 min to stop the reaction. Twenty microliters of each samples was subjected to HPLC analysis as described in Materials and Methods. The elution times of the standards arabinose (1), arabinobiose (2), arabinotriose (3), and arabinotetraose (4) are indicated.