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Negative image of ethidium bromide-stained DGGE gel containing PCR-amplified segments of 16S rRNA genes obtained by using universal bacterial primers. Lanes E, M, and H correspond to the aerobic epilimnion, the oxic-anoxic metalimnion, and the anaerobic rich-sulfide hypolimnion, respectively. Small circles correspond to the bands excised from the gel and then sequenced, and numbers are given for these sequences.
