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. 2005 Sep 30;1(1):e4. doi: 10.1371/journal.ppat.0010004

Figure 2. PB1-F2 Protein-Mediated Enhancement of TNFα-Induced Apoptosis Is Inhibited by Bcl-xL.

Figure 2

A549 cells containing a stably-integrated neomycin resistance gene (A549-neo) (A), or A549 cells stably overexpressing Bcl-xL (A549-Bcl-xL) (B) were transfected with either empty vector or vector encoding HA-tagged PB1-F2. At 24 h post-transfection, cells were treated with 50 ng/ml TNFα, where indicated. Then 6 h post-treatment, the cells were fixed and stained with M30 antibody to cleaved cytokeratin and with anti-HA antibody.