TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristics | Source |
|---|---|---|
| Strains | ||
| L. monocytogenes EGD | Laboratory stock | |
| L. monocytogenes ED1 (rel::Tn917 lx) | This study | |
| L. monocytogenes ED1/pMK4Em | This study | |
| L. monocytogenes ED1/pMK4Emrel | This study | |
| E. coli DH5α | Toyobo | |
| E. coli MC1061 | Laboratory stock | |
| Plasmids | ||
| pDlux917 | Apr Cmr Emr in E. coli and Cmr Emr in gram-positive bacteria | Shih-Tung Liu |
| pMK4 | Apr Cmr in E. coli and Cmr in gram-positive bacteria | A. Bubert (28) |
| pUC19 | Apr | Takara |
| pMK4Em | Apr Cmr Emr in E. coli and Cmr Emr in gram-positive bacteria; constructed with pMK4 BamHI digest and Emr fragment from pBR322::MudEm BamHI digest | This study |