TABLE 1.
Strain or plasmid | Characteristics | Source |
---|---|---|
Strains | ||
L. monocytogenes EGD | Laboratory stock | |
L. monocytogenes ED1 (rel::Tn917 lx) | This study | |
L. monocytogenes ED1/pMK4Em | This study | |
L. monocytogenes ED1/pMK4Emrel | This study | |
E. coli DH5α | Toyobo | |
E. coli MC1061 | Laboratory stock | |
Plasmids | ||
pDlux917 | Apr Cmr Emr in E. coli and Cmr Emr in gram-positive bacteria | Shih-Tung Liu |
pMK4 | Apr Cmr in E. coli and Cmr in gram-positive bacteria | A. Bubert (28) |
pUC19 | Apr | Takara |
pMK4Em | Apr Cmr Emr in E. coli and Cmr Emr in gram-positive bacteria; constructed with pMK4 BamHI digest and Emr fragment from pBR322::MudEm BamHI digest | This study |