FIG. 3.

Detection of SNPs at selected diagnostic positions for genotype II isolates Iowa and GCH1. The letters above the probe positions indicate the polymorphic nucleotides for each probe combination. Probes in italics are the perfectly matched probes for genotype II isolates (see Table 1 for the complete probe sequences). Results were based on a minimum of two replicates for each isolate. Error bars represent ±2 standard errors of the mean. All hybridizations were conducted by using the Cy3 labeling strategy and overnight hybridization at 4°C, except for panel D, where the hybridization temperature was room temperature (ca. 22°C). (A) Positions 1368 and 1371. For clarity, only the probes with the three greatest values are shown. (B) Position 1404. T′ is a single-base-pair mismatch between the perfectly matched probe, m4, and the probe for C. felis. This polymorphism occurs at position 1398 (numbering based on GenBank accession number AF221535). (C) Position 1533. (D) Position 1542. OD, optical density.