FIG. 4.

Comparison of hybridization results for genotype I isolates TU502 and UG502 at position 1419. No hybridization signal was detected for 1419 m12 and m13, which are omitted from this graph for clarity. Probe 1419 m1 is the perfectly matched probe for genotype I isolates and the only position of relevance for the genotype I SNP code. Letters above the probes indicate the polymorphic nucleotides at positions 1419 and 1422, respectively. Probes m8 to m13 are outlier probes containing multiple polymorphisms at positions other than and in addition to 1419 and 1422. Results were based on a minimum of two replicates for each isolate. Error bars represent ±2 standard errors of the mean. Hybridizations were conducted by using the Cy3 labeling strategy and overnight hybridization at 4°C. Single-nucleotide mismatch could not be reliably achieved for genotype II isolates when the m13 probe was the perfectly matched probe for these isolates. OD, optical density.