TABLE 1.

Sequences of the microarray capture probes

Probe name	Probe sequencea	Tm (°C)b	Sequence host specificity
1368 m1	5′-TCA-AAA-GTA-ACT-TCA-3′	38	C. parvum genotype I (AF 221535 sequence)
1368 m2	5′-TCA-AAT-GTA-ACT-TCA-3′	38	SNP variant at position 1371
1368 m3	5′-TCA-AAC-GTA-ACT-TCA-3′	40	SNP variant at position 1371
1368 m4	5′-TCA-AAG-GTA-ACT-TCA-3′	40	SNP variant at position 1371
1368 m5	5′-TCA-AAA-GTT-ACT-TCA-3′	38	C. muris (AF 221542 sequence)
1368 m6	5′-TCA-AAA-GTG-ACT-TCA-3′	40	C. parvum pig, monkey, and ferret isolates; C. wrairi, C. meleagridis
1368 m7	5′-TCA-AAA-GTC-ACT-TCA-3′	40	C. muris (AF 221543 sequence)
1368 m8	5′-TCA-AAT-GTT-ACC-TCA-3′	40	C. baileyi
1368 m9	5′-TCA-AAA-GTT-ACC-TCA-3′	40	C. serpentis
1368 m10	5′-TCA-AAG-GTG-ACC-TCA-3′	44	C. parvum dog isolate
1368 m11	5′-TCA-AAA-GTA-ACC-TCA-3′	40	Cryptosporidium sp. desert monitor isolate
1368 m12	5′-TCG-AAG-GTT-ACC-TCG-3′	46	C. felis
1368 m13	5′-TCA-AAG-GTG-ACT-TCA-3′	42	C. parvum genotype II isolates
1368 m14	5′-TCA-AAA-GTC-ACT-TCA-3′	40	C. muris (AF 221543 sequence)
1404 m1	5′-CAG-CAG-ATA-CAT-TCA-3′	42	C. parvum genotype I, pig, mouse, and ferret isolates; C. wrairi, C. meleagridis
1404 m2	5′-CAG-CAG-AAA-CAT-TCA-3′	42	SNP variant at position 1404
1404 m3	5′-CAG-CAG-AGA-CAT-TCA-3′	44	SNP variant at position 1404
1404 m4	5′-CAG-CAG-ACA-CAT-TCA-3′	44	C. parvum genotype II isolates
1404 m5	5′-CTG-CTG-ATA-CAT-TTA-3′	40	C. muris (AF 221542 sequence)
1404 m6	5′-CAG-CAG-ACA-CAT-TTA-3′	42	C. felis
1404 m7	5′-CTG-CCG-AAA-CAT-TTA-3′	42	C. serpentis
1404 m8	5′-CAG-CAG-AAA-CAT-TTA-3′	40	C. baileyi
1404 m9	5′-CGG-CGG-AAA-CAT-TCA-3′	46	C. parvum dog isolate
1419 m1	5′-CCA-GTG-CTT-TTA-TCA-3′	42	C. parvum genotype I isolates
1419 m2	5′-CCA-GTA-CTT-TTA-TCA-3′	40	SNP variant at position 1422
1419 m3	5′-CCA-GTC-CTT-TTA-TCA-3′	42	SNP variant at position 1422
1419 m4	5′-CCA-GTT-CTT-TTA-TCA-3′	40	SNP variant at position 1422
1419 m5	5′-CCA-GTG-CTA-TTA-TCA-3′	42	SNP variant at position 1419
1419 m6	5′-CCA-GTG-CTC-TTA-TCA-3′	44	C. meleagridis
1419 m7	5′-CCA-GTG-CTG-TTA-TCA-3′	44	SNP variant at position 1419
1419 m8	5′-CCA-GTA-CTT-TTA-TCT-3′	42	C. parvum pig isolate
1419 m9	5′-CCG-GTG-CTG-TTG-TCA-3′	48	C. parvum dog isolate
1419 m10	5′-CCA-GTG-CTC-TTG-TCA-3′	46	C. felis
1419 m11	5′-CCG-GTA-CTC-TTG-TCA-3′	46	C. muris (AF 221542 and AF 221543 sequences)
1419 m12	5′-CCG-GTA-CTC-TTA-TCA-3′	44	C. serpentis
1419 m13	5′-CCA-GTA-CTC-TTA-TCA-3′	42	C. parvum genotype II and ferret isolates; C. baileyi, C. wrairi, desert monitor isolate
1464 m1	5′-ATA-ATC-TAC-CCT-TAT-3′	38	C. parvum genotype I and II isolates, C. parvum pig and ferret isolates, C. meleagridis, desert monitor isolate
1464 m2	5′-ATA-ATC-TTC-CCT-TAT-3′	38	SNP variant at position 1464
1464 m3	5′-ATA-ATC-TGC-CCT-TAT-3′	40	C. parvum mouse isolate
1464 m4	5′-ATA-ATC-TCC-CCT-TAT-3′	40	SNP variant at position 1464
1464 m5	5′-AGA-GCC-TTC-CCT-TGA-3′	46	Does not match any Cryptosporidium sequences based on this alignment
1464 m6	5′-ATA-AAC-GAC-CTT-TAT-3′	38	C. muris (AF 221542 and AF 221543 sequences), C. serpentis
1464 m7	5′-ATA-AAC-GAC-CCT-TAT-3′	40	C. baileyi
1479 m1	5′-CAA-TAT-CGT-CCT-TTG-3′	42	C. parvum genotype I, II, and ferret isolates; C. wrairi, C. meleagridis
1479 m2	5′-CAA-TAT-CAT-CCT-TTG-3′	40	SNP variant at position 1479
1479 m3	5′-CAA-TAT-CTT-CCT-TTG-3′	40	C. muris (AF 221542 and AF 221543 sequences), C. serpentis
1479 m4	5′-CAA-TAT-CCT-CCT-TTG-3′	42	SNP variant at position 1479
1479 m5	5′-CGA-TAT-CAT-CCT-TTG-3′	42	C. parvum pig isolate
1479 m6	5′-CGA-TAT-CGT-CCT-TGG-3′	46	C. parvum dog isolate
1479 m7	5′-CAA-TAT-CTT-CTT-TAG-3′	38	C. baileyi
1479 m8	5′-CGA-TAT-CTT-CCT-TAG-3′	42	C. felis
1479 m9	5′-CAA-TAT-CAT-CCT-TAC-3′	40	Does not match any Cryptosporidium sequences based on this alignment
1533 m1	5′-GTC-TGT-TTT-GCT-CAT-3′	42	C. parvum human (AF 221535 sequence), mouse, and ferret isolates; C. wrairi
1533 m2	5′-GTC-TGT-TAT-GCT-CAT-3′	42	SNP variant at position 1533
1533 m3	5′-GTC-TGT-TGT-GCT-CAT-3′	44	C. parvum monkey isolate (AF 221534)
1533 m4	5′-GTC-TGT-TCT-GCT-CAT-3′	44	C. parvum genotype II isolates
1533 m5	5′-GTT-TAT-TTT-GTT-CAT-3′	36	Cryptosporidium sp. (desert monitor isolate)
1533 m6	5′-GTC-TAT-TTT-GTT-CAT-3′	38	C. parvum pig isolate
1533 m7	5′-TAC-GAT-TTT-GTT-CAT-3′	36	C. baileyi
1533 m8	5′-CAC-GAT-TCT-GTT-CAT-3′	42	C. muris (AF 221542 and AF 221543), C. serpentis
1542 m1	5′-CAA-TCT-TGA-GTC-TGT-3′	42	C. parvum genotype I, mouse, and ferret isolates; C. wrairi, C. meleagridis
1542 m2	5′-CAA-TCT-TAA-GTC-TGT-3′	40	C. parvum genotype II isolates
1542 m3	5′-CAA-TCT-TTA-GTC-TGT-3′	40	SNP variant at position 1542
1542 m4	5′-CAA-TCT-TCA-GTC-TGT-3′	42	SNP variant at position 1542
1542 m5	5′-CAA-TCT-TGA-GTT-TAT-3′	38	Cryptosporidium sp. (desert monitor isolate)
1542 m6	5′-CAA-TCT-TGA-TAC-GAT-3′	40	C. baileyi
1542 m7	5′-CAA-TCT-TTT-CAC-GAT-3′	40	C. muris (AF 221542 sequence), C. serpentis
1542 m8	5′-CAA-TCT-TCT-CAC-GAT-3′	42	C. muris (AF 221543 sequence)

^aBoldface indicates sequence polymorphisms between isolates and species.

^bCalculated by the 2(A+T) + 4(G+C) method.