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. 2002 Apr;68(4):1962–1971. doi: 10.1128/AEM.68.4.1962-1971.2002

TABLE 2.

Induction of green fluorescence in P. fluorescens A506(pTS) by various compoundsa

Compound Induction ratio (mean ± SEM)
Benzene derivatives
    Ethylbenzene 14.7 ± 0.8
    Toluene 14.2 ± 0.5
    Chlorobenzene 11.3 ± 3.0
    Benzene 11.0 ± 0.7
    Styrene 10.4 ± 1.0
    Phenol 2.8 ± 0.1
    Isopropyl benzene 2.6 ± 0.3
    Propyl benzene 2.2 ± 0.4
    o-Xylene 2.4 ± 0.2
    m-Xylene 1.3 ± 0.1
    p-Xylene 1.4 ± 0.3
    2-Hydroxy benzoic acid 1.5 ± 0.6
    Benzoate 1.1 ± 0.1
    2,4-Dinitrophenol 0.6 ± 0.1
Aliphatic alcohols
    Ethanol 1.0 ± 0.1
    Isopropanol 1.0 ± 0.1
    Butanol 1.0 ± 0.1
    2-Methyl-1-propanol 0.9 ± 0.1
Alkenes and alkanes
    TCE 12.2 ± 1.3
    1,2-cis-DCE 8.7 ± 0.3
    1,1-DCE 6.7 ± 0.6
    1,2-trans-DCE 1.6 ± 0.3
    1,1,1-TCE 1.1 ± 0.2
    2-Methyl-2-butene 6.5 ± 1.0
    n-Hexane 0.9 ± 0.1
    Ethylene glycol 1.1 ± 0.1
    Cyclohexane 1.2 ± 0.1
    Isopentane 1.3 ± 0.3
Polyaromatics
    Naphthalene 1.2 ± 0.1
    Phenanthrene 1.1 ± 0.1
    Biphenyl 1.0 ± 0.3
Other
    Catechol 1.1 ± 0.1
    Methyl-tert-butyl ether 0.8 ± 0.1
No effector control 1.0
a

Induction ratio is the RFU of an effector-exposed sample divided by the RFU of a no-effector control; no-effector control was arbitrarily set to 1.0. Assay concentrations of effectors were 80 mg ml−1, except for saturated aqueous solutions of phenanthrene (approximately 1 mg liter−1), naphthalene (approximately 10 mg liter−1), biphenyl (approximately 7.5 mg liter−1), n-hexane (approximately 1 mg ml−1), cyclohexane (approximately 55 mg liter−1), equimolar (610 μM) concentrations of the chloroethylenes, 2-methyl-2-butene, isopentane, isopropylbenzene, and propylbenzene (200 μM). All experiments were repeated 3 to 5 times. Values in bold indicate that there was a statistically significant increase (P < 0.05) in fluorescence above that of the no-effector control based on a paired t test.