TABLE 4.
Microscopic morphology during chemostat cultivation of A. oryzae ChsB/G and NiiA1 strainsa
| Parameter | Value for strain:
|
|||||
|---|---|---|---|---|---|---|
| A1560 | ChsB/G | NiiA1 | ||||
| Time after nitrogen source shift (h) | ND | 0b | 66c | 336 | 407d | |
| Time after dilution rate step-up (h) | 0 | 71 | ||||
| Specific growth rate (h−1) | 0.10 | 0.10 | 0.10 | 0.10 | 0.10 | 0.16 |
| Nitrogen source | NH4+ | NH4+ | NO3− | NH4+ | NH4+ | NH4+ |
| lHGU (μm tip−1 ± 95% confidence interval) | 130 ± 14 | 83 ± 6 | 133 ± 9 | 112 ± 9 | 92 ± 7 | 87 ± 6 |
a
Two control experiments with the wild-type, A1560, and ChsB/G strains show the length of the hyphal growth unit, lHGU, with these strains. For the NiiA1 strain, at time zero the culture was in steady state growing on NO3−. At zero hours, the nitrogen source was shifted to NH4+. At the time points indicated, RNA was extracted, subjected to Northern blotting, and hybridized to probes specific for chsB and the control, tpi.
b
Northern blot sample 1.
c
Northern blot sample 2.
d
Northern blot sample 3.