TABLE 1.
Comparison of specificities of various amylolytic enzymes
| Substrate | Initial velocitya
|
|||||||
|---|---|---|---|---|---|---|---|---|
| NPL | CDase | BLMA | BMNP | BSA | BLA | NOV | β-AMY | |
| Potato amylose | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 |
| Amylose AS-1000 | 114.3 | 122.9 | 140.6 | 106.6 | 116.5 | 95.5 | 66.1 | 14.9 |
| Amylose AS-320 | 106.1 | 130.2 | 130.4 | 96.6 | 101.9 | 82.0 | 64.6 | 26.4 |
| Amylose AS-110 | 119.8 | 148.6 | 154.6 | 89.1 | 106.6 | 85.3 | 65.0 | 65.8 |
| Amylose AS-70 | 133.1 | 155.4 | 153.0 | 89.8 | 112.1 | 90.1 | 65.4 | 98.0 |
| Amylose EX-III | 161.7 | 212.0 | 232.6 | 85.1 | 107.9 | 85.8 | 89.8 | 138.6 |
| Amylose EX-I | 194.2 | 265.6 | 261.7 | 60.3 | 66.6 | 56.5 | 80.5 | 143.6 |
| Maltoheptaose | 117.1 | 173.1 | 291.0 | 23.8 | 19.6 | 44.7 | 67.1 | 38.0 |
| Maltohexaose | 115.3 | 141.5 | 254.3 | 23.5 | 22.2 | 13.9 | 64.3 | 42.6 |
| Maltopentaose | 107.0 | 178.1 | 246.1 | 13.6 | 13.3 | 8.2 | 61.1 | 29.6 |
| Maltotetraose | 67.4 | 87.9 | 171.4 | 14.6 | 8.6 | 11.2 | 35.2 | 22.5 |
| Maltotriose | 24.9 | 69.9 | 46.7 | NDb | ND | ND | 22.6 | ND |
| Potato amylopectin | ND | ND | ND | 74.9 | 83.5 | 76.4 | 115.9 | 85.9 |
| Waxy maize starch | ND | ND | ND | 61.9 | 76.5 | 78.6 | 129.6 | 114.2 |
| Higly branched cyclic dextrin | 14.9 | ND | ND | 57.7 | 74.2 | 70.0 | 127.1 | 98.5 |
| Potato starch | 31.9 | 10.8 | 26.0 | 59.5 | 77.2 | 66.5 | 92.5 | 62.4 |
| α-Cyclomaltodextrin | 413.7 | 400.5 | 568.9 | ND | ND | ND | ND | ND |
| β-Cyclomaltodextrin | 401.8 | 481.6 | 449.8 | 18.2 | ND | ND | ND | ND |
| γ-Cyclomaltodextrin | 379.9 | 596.7 | 526.4 | 53.8 | 55.3 | ND | ND | ND |
| Pullulan | 59.0 | 7.0 | ND | 9.5 | ND | ND | ND | ND |
a
The initial velocity is indicated with respect to that for potato amylose, which was defined as 100. NPL, B. stearothermophilus neopullulanase; CDase, cyclomaltodextrinase; BLMA, B. licheniformis maltogenic amylase; BMNP, B. megaterium neopullulanase; BSA, bacterial saccharifing α-amylase; BLA, bacterial liquefying α-amylase; NOV, Novamyl; β-AMY, soybean β-amylase.
b
ND, enzyme activity was not detectable (the initial velocity was less than 1/100 of that for potato amylose).