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. 2002 Apr;68(4):1907–1913. doi: 10.1128/AEM.68.4.1907-1913.2002

FIG. 4.

FIG. 4.

pH optimum engineering of A. fumigatus and consensus phytase based on a comparison with Peniophora lycii phytase. (A and B) pH activity profiles with phytic acid as substrate; (C and D) pH activity profiles with p-nitrophenyl phosphate as substrate; (E and F) specific activities at pH 5.0 with 5 mM concentrations of a series of phosphate compounds (see legend to Fig. 2). (A, C, and E) A. fumigatus Q27T phytase (▪) and A. fumigatus Q27T K68A phytase (□); (B, D, and F) consensus phytase-10 thermo[3] Q27T (•) and consensus phytase-10 thermo[3] Q27T K68A (○).