TABLE 1.

Comparison of PAH mineralization tests when the WST-1 microtiter plate method and a ¹⁴C-PAH mineralization method were used

Strain	No PAH: WST-1 absorbancea	Fluorene		Phenanthrene		Anthracene		Fluoranthene		Pyrene
		14C %b	WST-1 absorbancea	14C %b	WST-1 absorbancea	14C %b	WST-1 absorbancea	14C %b	WST-1 absorbancea	14C %b	WST-1 absorbancea
EPA505	0.00 ± 0.00	0.1	0.00 ± 0.00	62.2	0.88 ± 0.25	11.1	0.00 ± 0.01	62.0	2.07 ± 0.24	0.2	0.00 ± 0.02
LB126	0.00 ± 0.00	79.2	0.96 ± 0.13	0.0	0.00 ± 0.00	0.2	0.00 ± 0.00	0.0	0.00 ± 0.00	0.1	0.01 ± 0.00
CRE7	0.00 ± 0.00	0.1	0.00 ± 0.00	13.4c	0.00 ± 0.00c	0.5	0.00 ± 0.00	0.0	0.00 ± 0.00	0.3	0.00 ± 0.02
LB501T	0.14 ± 0.01	0.2	0.27 ± 0.09	45.3	2.09 ± 0.11	46.4	2.19 ± 0.29	52.6	2.03 ± 0.09	0.3c	2.02 ± 0.48c
VM451	0.10 ± 0.03	0.1	0.05 ± 0.01	44.1	1.36 ± 0.48	1.2	0.08 ± 0.01	44.7	1.40 ± 0.08	0.2c	1.58 ± 0.19c
VM455	0.06 ± 0.02	0.1	0.05 ± 0.00	36.0	0.76 ± 0.23	4.4	0.05 ± 0.01	54.3	1.96 ± 0.06	51.3	1.38 ± 0.13

^aChange in WST-1 formazan absorbance measured after 90 min (average ± 1 standard deviation; n = 4).

^bPercentage of ¹⁴C-labeled PAH mineralized to ¹⁴CO₂ (average of duplicate samples).

^cThe results obtained with the two assays are not consistent.