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Tm curve analysis of SYBR Green assay using real-time PCR. Tm was 81.8 and 83.8°C for stx1 (ATCC 43890) and stx2 (ATCC 43889), respectively. Sample 3 contained both stx1 and stx2 (C9490), and the Tm could be resolved between the two products. Sample 4 was a negative control (ATCC 43888).
