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Compatible solute accumulation by L. monocytogenes DP-L1044 (A) and mutant L. monocytogenes LTS4a (B) during balanced growth in modified Pine's medium under different conditions of osmotic and cold stress. Cultures were harvested and washed, and cytoplasmic contents were extracted with perchloric acid. Alanine (50 mM) was added to each extract as an internal standard, and compatible solutes were quantitated by natural-abundance 13C-NMR spectroscopy.
