TABLE 2.
Effect of potential electron donors, electron acceptors, and carbon sources on growth of strain HGM-K1a
| Electron donorb | Electron acceptor (concn) | Carbon source | Gas phase | Maximum cell yieldc |
|---|---|---|---|---|
| H2 | NO3− (10 mM) | CO2 + HCO3− | H2/CO2, 80/20; 300 kPa | ++ |
| O2 (20%) | CO2 + HCO3− | H2/CO2/O2, 65/15/20; 100 kPa | NG | |
| O2 (5%) | CO2 + HCO3− | H2/CO2/O2, 75/20/5; 100 kPa | ++ | |
| O2 (1%) | CO2 + HCO3− | H2/CO2/O2, 79/20/1; 100 kPa | ++ | |
| Ferric citrate (5 mM) | CO2 + HCO3− | H2/CO2, 80/20; 300 kPa | ++ | |
| Ferrihydrite (10 mM) | CO2 + HCO3− | H2/CO2, 80/20; 300 kPa | ++ | |
| HAsO42− (2 mM) | CO2 + HCO3− | H2/CO2, 80/20; 300 kPa | ++ | |
| SeO42− (2 mM) | CO2 + HCO3− | H2/CO2, 80/20; 300 kPa | ++ | |
| SeO32− (2 mM) | CO2 + HCO3− | H2/CO2, 80/20; 300 kPa | ++ | |
| None | CO2 + HCO3− | H2/CO2, 80/20; 300 kPa | NG | |
| None | Any shown above | CO2 + HCO3− | N2/CO2, 80/20; 300 kPa | NG |
| Organic substrates | Any shown above | Organic substrates | N2; 300 kPa | NG |
| H2 | Any shown above | Organic substrates | H2; 300 kPa | NG |
| Any shown above | CO2 + HCO3− + organic substrates | H2/CO2, 80/20; 300 kPa | ++ | |
| S0 or S2O32− | O2 (20%) | CO2 + HCO3− | N2/CO2/O2, 65/15/20; 100 kPa | ++ |
| O2 (5%) | CO2 + HCO3− | N2/CO2/O2, 75/20/5; 100 kPa | ++ | |
| O2 (1%) | CO2 + HCO3− | N2/CO2/O2, 79/20/1; 100 kPa | ++ | |
| NO3− (10 mM) | CO2 + HCO3− | Ar/CO2, 80/20; 300 kPa | ++ | |
| Ferric citrate (5 mM) | CO2 + HCO3− | N2/CO2, 80/20; 300 kPa | + | |
| Ferrihydrite (10 mM) | CO2 + HCO3− | N2/CO2, 80/20; 300 kPa | + | |
| HAsO42− (2 mM) | CO2 + HCO3− | N2/CO2, 80/20; 300 kPa | + | |
| SeO42− (2 mM) | CO2 + HCO3− | N2/CO2, 80/20; 300 kPa | + | |
| SeO32− (2 mM) | CO2 + HCO3− | N2/CO2, 80/20; 300 kPa | + | |
| Any shown above | Organic substrates | N2; 300 kPa | NG | |
| None | CO2 + HCO3− | N2/CO2, 80/20; 300 kPa | NG |
a
These experiments were conducted with media containing various combinations of electron donors, electron acceptors, carbon sources, and gas phases instead of those in the mjANHOX-NO3 medium.
b
Yeast extract (0.02%), tryptone (0.02%), peptone (0.02%), Casamino Acids (0.02%), and starch (0.02%) were tested as organic substrates. The concentrations of elemental sulfur and thiosulfate added were 3% and 10 mM, respectively.
c
Maximum cell yield is indicated as follows: ++, >2.0 × 108 cells ml−1; +, <2.0 × 108 cells ml−1 but >7.0 × 107 cells ml−1; NG, no growth.