FIG. 4.

Nucleotide pools in MBP71 grown with or without thymidine. For each of the triphosphate nucleotide spot designations the “TP” part of the name has been omitted, so that, e.g., dTTP is referred to as dT. (A) Triphosphate nucleotide pool for MBP71 grown in LSA medium with thymidine. (B) Same as panel A except that thymidine was omitted. The various spots were identified from earlier work (27). Spot labels: Total, 1/10 of the total amount of activity run on each plate; PP, pyrophosphate; PRPP, 5-phosphoribosyl-1-pyrophosphate; ppGpp, guanosine 5′-diphosphate 3′-diphosphate.