TABLE 1.
Bacteria, phages, and plasmids used in this research
| Bacterium, phage, or plasmid | Comment | Reference or source |
|---|---|---|
| Bacteria | ||
| Lactococcus lactis subsp. lactis | ||
| NCDO712 | Wild type | 20 |
| CHCC373 | Wild type | Chr. Hansen Culture Collectiona |
| MBP65-3 | CHCC373 with pMBP63 integrated | This study |
| MBP71 | CHCC373 with ΔthyA | This study |
| Escherichia coli DH5α | Life Technologies Inc. | |
| Phages | ||
| CHPC12 | P335 species | Chr. Hansen Phage Collection |
| CHPC412 | P335 species | Chr. Hansen Phage Collection |
| CHPC708 | 936 species | Chr. Hansen Phage Collection |
| CHPC710 | 936 species | Chr. Hansen Phage Collection |
| CHPC733 | P335 species | Chr. Hansen Phage Collection |
| CHPC783 | 936 species | Chr. Hansen Phage Collection |
| CHPC795 | 936 species | Chr. Hansen Phage Collection |
| CHPC814 | 936 species | Chr. Hansen Phage Collection |
| CHPC836 | 936 species | Chr. Hansen Phage Collection |
| Plasmids | ||
| pGhost9 | Gene inactivation vector | 36 |
| pMBP57 | pGhost9 derivative with 400 bp of thyA | This study |
| pMBP63 | pGhost9 derivative with ΔthyA (two 800-bp fragments) | This study |
a
Chr. Hansen A/S, Hørsholm, Denmark.