TABLE 3.
Product patterns of CHCC373 and MBP71a
| Strain | Temp (°C) | Inoculum (%) | Concn of added thymidine (mg/liter) | pH | Acid (%) | Mean lactic acid concn ± SD (g/liter) | Mean ethanol concn ± SD (mg/liter) | Mean acetaldehyde concn ± SD (mg/liter) |
|---|---|---|---|---|---|---|---|---|
| CHCC373 | 30 | 1 | 4.5 | 204 | 6.1 ± 0.1 | 32 ± 2 | 2.6 ± 0.3 | |
| 37 | 1 | 4.5 | 204 | 6.0 ± 0.0 | 32 ± 3 | 2.3 ± 0.4 | ||
| MBP71 | 30 | 20 | 5.2 | 112 | 3.2 ± 0.0 | 28 ± 1 | 4.0 ± 0.2 | |
| 37 | 10 | 5.3 | 100 | 2.8 ± 0.3 | 32 ± 1 | 3.4 ± 0.2 | ||
| 30 | 1 | 20 | 4.8 | 162 | 5.1 ± 0.1 | 45 ± 2 | 4.3 ± 0.5 | |
| 37 | 1 | 20 | 4.7 | 177 | 5.0 ± 0.1 | 65 ± 4 | 5.0 ± 0.5 | |
| 37 | 5 | 0.5 | 5.3 | 100 | 2.5 | 76 ± 3 | 4.7 ± 0.4 | |
| 37 | 5 | 1.0 | 5.0 | 138 | 3.6 ± 0.1 | 102 ± 1 | 5.4 ± 0.1 |
a
MBP71 and CHCC373 were inoculated at various levels into 200 ml of RSM with various amounts of thymidine as indicated. The cultures were set up at the indicated temperature, as were two flasks with no cells added. At 10 h two samples were taken from each culture, and the end product concentrations were determined. The standard deviation is shown for each value, except for one value, for which a sample was lost. The pH at 10 h is also shown, and so is the amount of acid produced, based on a titration curve of RSM with HCl, relative to the highest observed pH, i.e., 5.3.