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Cytotoxocity of purified SAP against P. yezoensis cells and HDF. (A) After incubation with 700 μg of SAP per ml at 25°C, thallus cells of P. yezoensis were stained with Evans blue, and the percentage of surviving cells was determined. (B) HDF cells were incubated with each concentration of SAP at 37°C for 12 h, and the growth rate of the cells was evaluated by the MTT assay. The data points and error bars represent means and standard deviations for triplicate experiments.
