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Purification of SAP from the culture supernatant of Streptomyces sp. strain AP77
| Purification step | Vol (ml) | Protein (mg) | Total activity (103 U) | Sp act (U/mg) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|---|
| Culture supernatant | 10,000 | 3,410 | 250 | 73 | 1.0 | 100 |
| Ultrafiltration | 500 | 234 | 180 | 769 | 10.5 | 72 |
| (NH4)2SO4 precipitation and dialysis | 20 | 191 | 14.7 | 77 | 1.1 | 5.9 |
| Ion exchange | 10 | 13.0 | 13.0 | 1,000 | 13.7 | 5.2 |
| Gel filtration | 10 | 3.2 | 5.4 | 1,688 | 23.1 | 2.2 |
| Native PAGE | 10 | 0.9 | 2.2 | 2,444 | 33.5 | 0.9 |
