FIG. 1.

The effect of Mn²⁺ amendment (73 ppm) on peroxidase gene expression of P. ostreatus grown under SSF conditions on cotton stalks. Gene expression was determined by relative RT-PCR utilizing specific primer sets for each gene. β-Tubulin gene expression was used as an internal control. Amplicons with increasing amounts (1-, 1.3-, and 1.5-fold amounts of RNA in panels a, b, and c, respectively) of mRNA template per gram of substrate, established on the basis of the linear range of the β-tubulin amplicon produced (see reference 9), are shown.