Table 5.
Number of replicate extract C. cayetanensis detections from sludge samples, mean percent recovery and standard deviation, number of inhibited extracts, and mean difference in internal amplification control Cq values for inhibited samples.
| Sludge type | Extraction method | Seed quantity | N | Detects | Mean % recovery (SDV) | Inhibited extracts (%) | Mean IAC Cq difference for inhibited extracts |
|---|---|---|---|---|---|---|---|
| RAS | PowerSoil | 1000 | 18 | 16 | 0.17 (0.13) | 15 (83) | 6.65 |
| PowerViral | 1000 | 9 | 9 | 28 (5) | 1 (11) | 3.16 | |
| PowerViral | 100 | 9 | 9 | 17 (13) | 4 (44) | 6.42 | |
| UNEX | 1000 | 9 | 9 | 10 (2) | 2 (22) | 3.32 | |
| UNEX | 100 | 9 | 9 | 4 (3) | 3 (33) | 5.85 | |
| REC | PowerSoil | 1000 | 12 | 12 | 1.1 (0.6) | 7 (58) | 5.07 |
| PowerViral | 1000 | 9 | 9 | 32 (7) | 0 | NAa | |
| PowerViral | 100 | 9 | 9 | 36 (9) | 0 | NA | |
| UNEX | 1000 | 9 | 9 | 11 (3) | 0 | NA | |
| UNEX | 100 | 9 | 9 | 12 (4) | 1 (11) | 3.12 |
a
NA: not applicable; no inhibition observed in these extracts.