TABLE 2.
Influence of different reagents on PalI activitya
| Reagent | Concn (mM) | PalI activity (%) |
|---|---|---|
| EDTA | 3 | 64.0 |
| 15 | 30.4 | |
| SDS | 3 | 0 |
| FeCl3 | 5 | 0 |
| FeCl2 | 1 | 75.3 |
| 5 | 48.6 | |
| LiCl2 | 5 | 93.7 |
| CaCl2 | 5 | 90.4 |
| HgCl2 | 1 | 0 |
| MgSO4 | 5 | 124.6 |
| ZnSO4 | 5 | 102.5 |
| CuSO4 | 1 | 85.6 |
| 5 | 76.9 | |
| MnCl2 | 1 | 126.7 |
| 5 | 145.7 |
a
Purified PalI protein was extensively dialyzed against 50 mM citrate-phosphate buffer (pH 6.0). The enzyme (2 μg in a 200-μl final volume) was preincubated with the indicated reagents and ions at room temperature for 10 min, and 20 μl of sample was taken for enzyme assay as described in Materials and Methods.