FIG. 1.
Colony PCRs comparing MR-1 and ALTA1. Lanes 1 and 2, reactions with primers C1 and C2 (Table 2) specific for the altA gene; lanes 3 to 5, reactions with primers R1 and R2 (Table 2) specific for the chloramphenicol acetyltransferase gene (cat) from pEP185.2; lanes 6 and 7, reactions with primer K1 (Table 2) specific for both ends of the kanamycin resistance (Kmr) gene. The templates for PCR were as follows: lanes 1, 3, and 6, MR-1; lanes 2, 4, and 7, ALTA1; and lane 5, pEP185.2. The sizes of the DNA markers (lane M) are indicated on the left.