TABLE 1.
Protein expression in B. cereus DL5 planktonic, PGW, and biofilm cells following 2 and 18 h of culturing in the absence and presence of glass wool
| Protein expression | No. of spots | % of total spots (n = 345) |
|---|---|---|
| Proteins unique to a specific growth phase | ||
| Planktonic cells (2 h) | 1 | 0.3 |
| PGW cells (2 h) | 2 | 0.6 |
| Biofilm cells (2 h) | 15 | 4.4 |
| Planktonic cells (18 h) | 1 | 0.3 |
| PGW cells (18 h) | 7 | 2.3 |
| Biofilm cells (18 h) | 7 | 2.0 |
| Proteins up-regulated in a specific phase compared with the levels of the corresponding spot in other phases | ||
| Planktonic cells (2 h) | 3 | 0.9 |
| PGW cells (2 h) | 10 | 2.9 |
| Biofilm cells (2 h) | 4 | 1.2 |
| Planktonic cells (18 h) | 7 | 2.0 |
| PGW cells (18 h) | 30 | 8.7 |
| Biofilm cells (18 h) | 19 | 5.5 |
| Proteins present in a specific phase regardless of time (2 h or 18 h) | ||
| Planktonic cells | 0 | 0 |
| PGW cells | 1 | 0.3 |
| Biofilm cells | 5 | 1.5 |
| Proteins present in all phases and always at the same level (no up- or down-regulation) | 35 | 10.1 |
| Proteins present in planktonic and PGW cells and absent in biofilm cells | 11 | 3.2 |
| Proteins present in PGW and biofilm cells and absent in planktonic cells | 0 | 0 |
| Proteins present in planktonic and biofilm cells and absent in PGW cells | 0 | 0 |
| After 2 h present only in biofilm cells; after 18 h present in PGW and biofilm cells | 2 | 0.6 |
| After 2 h present only in planktonic cells; after 18 h present in planktonic and PGW cells | 1 | 0.4 |