TABLE 2.
Growth phase-related differences in the levels of expression of proteins selected for N-terminal protein sequencing
| Protein spot | Level of expression
|
|||||
|---|---|---|---|---|---|---|
| Planktonic cells (2 h) | PGW cells (2 h) | Biofilm cells (2 h) | Planktonic cells (18 h) | PGW cells (18 h) | Biofilm cells (18 h) | |
| B5a | 0a | 0 | 0 | −2 | −1 | −1 |
| B11b | 0 | 1 | 2 | 0 | 2 | 0 |
| D5h | 0 | 0 | 0 | −1 | 3 | 3 |
| E1d | 0 | 0 | 0 | 0 | 1 | 1 |
| E7g | Ab | A | 0 | A | 0 | 0 |
| E7h | 0 | 0 | 3 | 0 | 3 | 3 |
| E9p | A | A | A | A | A | Uc |
| G1b | 0 | 1 | 2 | 2 | 3 | 3 |
a
A value of 0 indicates a level of synthesis similar to that of the corresponding spot in the 2-h planktonic growth phase (reference spot intensity). A positive value indicates up-regulation, and a negative value indicates repression or down-regulation.
b
A, protein spot is absent.
c
U, protein spot is unique to the growth phase.